KMID : 0604520160420030217
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Journal of the Society of Cosmetic Scientists of Korea 2016 Volume.42 No. 3 p.217 ~ p.226
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Antioxidant and Cellular Protective Effects of Moringa oleifera Leaves Extract
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Xuan Song Hua
Kim A-Rang Jeong Yoon-Ju Lee Nan-Hee Park Soo-Nam
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Abstract
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In this study, we investigated the antioxidative and cellular protective effects on HaCaT cells and erythrocytes of Moringa oleifera (M. oleifera) leaves extract and its fractions. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction of M. oleifera leaves. The free radical scavenging activity (FSC50) of the extract and fractions of M. oleifera leaves were in the following order: 50% ethanol extract (77.10 ¥ìg/mL) < ethyl acetate fraction (20.63 ¥ìg/mL) < aglycone fraction (17.00 ¥ìg/mL) by using the 1, 1-diphenyl-2-picrylhydrazyl. In Fe3+-EDTA/H2O2 system using the luminol, reactive oxygen species (ROS) scavenging activities (total antioxidant capacity, OSC50) of aglycone fraction (OSC50 = 0.63 ¥ìg/mL) was the strongest among all extracts, which was much higher than L-ascorbic acid (1.50 ¥ìg/mL). In the 1O2-induced cellular damage of erythrocytes, the cellular protective effects of 50% ethanol extract (¥ó50 = 46.9 min) and aglycone fraction (¥ó50 = 122.1 min) were higher than (+)-¥á-tocopherol (¥ó50 = 37.7 min), known as a lipophilic antioxidant at 10 ¥ìg/mL. After cell damage induced by 400 mJ/cm2 UVB irradiation, the cellular protective effects of ethyl acetate and aglycone fraction of M. oleifera leaves extract were showed on the concentration from 0.20 to 1.56 ¥ìg/mL. These results suggest that M. oleifera leaves extract and its fractions can function as a natural antioxidant agent in cosmetics on skin exposed to UV radiation by protecting cellular membrane against ROS.
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KEYWORD
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M. oleifera, antioxidant, reactive oxygen species, cellular protective effect
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